Genomics/Sequencing Data

Sequencing data will be deposited to the National Center for Biotechnology Information’s Sequence Read Archive (SRA).

Please register 1) one project/study BioProject accession number and 2) individual BioSample submissions for each file. These steps are necessary to provide enough metadata about each sample within SRA and to link sequences back to metadata stored at BCO-DMO. While BCO-DMO does not host sequencing data, metadata about each file and links to the bioSample and SRA accession numbers should be submitted to BCO-DMO to support data reuse. For more information, please visit the Contributing Genetic Accessions page in the BCO-DMO "How To?" GitBook.

Overview directions for submitting to NCBI’s Sequence Read Archive (SRA)

1. Create an account or login to your NCBI account here.

2. Use NCBI’s Portal Wizard to create and register your BioProject and BioSample entries (containing metadata about each sequenced sample) following the prompts.

   1. Creating BioSamples can be time consuming. Please ask the Digital Coordinator and/or reach out to NCBI for assistance.

3. Create a new SRA submission (click on the ‘New submission’ button).

4. Submit SRA metadata - information that will link your project, samples/experiments and file names. Please refer to the SRA Metadata Overview.

5. Upload raw sequence data files. Please refer to SRA File Upload help.